| 04 Nov 06 | Sauchie Juniors | 1 | v | 2 | Hill of Beath Hawthorn | | |
| 04 Nov 06 | Kilsyth Rangers | 5 | v | 2 | Dunbar United | | |
| 04 Nov 06 | Blackburn United | 4 | v | 0 | Annbank United | | |
| 04 Nov 06 | Broughty Athletic | 4 | v | 1 | Troon | | |
| 04 Nov 06 | Lewis United | 0 | v | 4 | Culter | | |
| 04 Nov 06 | Arthurlie | 5 | v | 0 | Forfar West End | | |
| 04 Nov 06 | Coupar Angus | 1 | v | 3 | Newburgh | | |
| 04 Nov 06 | Arniston Rangers | 1 | v | 2 | Haddington Athletic | | |
| 04 Nov 06 | Benburb | 0 | v | 2 | Bo'ness United | | |
| 04 Nov 06 | Newmains United | 1 | v | 1 | Hurlford United | | Replay 4-3 to Newmains United |
| 04 Nov 06 | Kello Rovers | 3 | v | 3 | Longside | | Replay 4-1 to Longside |
| 04 Nov 06 | Musselburgh Athletic | 2 | v | 2 | Dundee Downfield | | Replay 2-1 to Musselburgh |
| 04 Nov 06 | Hillend | 3 | v | 1 | Johnstone Burgh | | |
| 04 Nov 06 | Lochee Harp | 1 | v | 0 | Rosyth Recreation | | |
| 04 Nov 06 | Bathgate Thistle | 1 | v | 1 | Carnoustie Panmure | | Replay 3-2 to Carnoustie Panmure |
| 04 Nov 06 | Blantyre Victoria | 2 | v | 0 | Maryhill | | |
| 04 Nov 06 | Glentanar | 3 | v | 0 | Darvel Juniors | | |
| 04 Nov 06 | Craigmark Burntonians | 1 | v | 1 | Banks O'Dee | | Banks O'Dee won replay 4-0. |
| 04 Nov 06 | Ashfield | 3 | v | 0 | Armadale Thistle | | |
| 04 Nov 06 | Port Glasgow Juniors | 1 | v | 0 | Cumnock Juniors | | |
| 04 Nov 06 | West Calder United | 0 | v | 5 | Harthill Royal | | |
| 04 Nov 06 | Irvine Victoria | 2 | v | 1 | Larkhall Thistle | | |
| 04 Nov 06 | Lochore Welfare | 1 | v | 4 | Pollok | | |
| 04 Nov 06 | Glenrothes Juniors | 2 | v | 2 | Bellshill Athletic | | Replay 3-0 to Bellshill |
| 04 Nov 06 | Shettleston | 0 | v | 3 | Auchinleck Talbot | | |
| 04 Nov 06 | Tayport | 4 | v | 2 | Thorniewood United | | |
| 04 Nov 06 | Forres Thistle | 0 | v | 1 | Luncarty | | |
| 04 Nov 06 | Dundee North End | 0 | v | 3 | Kilwinning Rangers | | |
| 04 Nov 06 | Whitburn Juniors | 1 | v | 0 | Shotts Bon Accord | | |
| 04 Nov 06 | Linlithgow Rose | 7 | v | 0 | Aberdeen Lads Club | | |
| 04 Nov 06 | Carluke Rovers | 4 | v | 2 | Greenock Juniors | | |
| 04 Nov 06 | Vale of Clyde | 4 | v | 1 | Lesmahagow Juniors | | |
| 04 Nov 06 | Lanark United | 0 | v | 0 | Glenafton Athletic | | Replay 2-1 to Lanark United |
| 04 Nov 06 | Kinnoull | 3 | v | 1 | Forth Wanderers | | |
| 04 Nov 06 | Largs Thistle | 1 | v | 2 | Neilston Juniors | | |
| 04 Nov 06 | Stonehaven | 3 | v | 2 | St. Andrew's United | | |
| 04 Nov 06 | Kelty Hearts | 14 | v | 0 | Burghead Thistle | | |
| 04 Nov 06 | Camelon Juniors | 1 | v | 0 | Dundee Violet | | |
| 04 Nov 06 | Strathspey Thistle | 0 | v | 2 | Arbroath Victoria | | |
| 04 Nov 06 | Islavale | 1 | v | 2 | Hermes | | |
| 04 Nov 06 | Bonnyrigg Rose Athletic | | - | | Cruden Bay | | |
| 04 Nov 06 | Banchory St. Ternan | 4 | v | 4 | Montrose Roselea | | Replay 1-0 to Montrose Roselea |
| 04 Nov 06 | Irvine Meadow XI | 2 | v | 1 | St. Anthony's | | |
| 04 Nov 06 | Kirkcaldy Y.M. | 2 | v | 1 | Formartine United | | |
| 04 Nov 06 | Jeanfield Swifts | 4 | v | 1 | Nairn St. Ninian | | |
| 04 Nov 06 | Oakley United | 8 | v | 0 | Girvan | | |
| 04 Nov 06 | Lochee United | 1 | v | 0 | Pumpherston | | |
| 04 Nov 06 | R.A.F. Lossiemouth | 0 | v | 5 | Ballingry Rovers | | |
| 04 Nov 06 | Penicuik Athletic | 5 | v | 2 | Fraserburgh United | | |
| 04 Nov 06 | Renfrew | 4 | v | 0 | Livingston United | | |
| 04 Nov 06 | Blairgowrie Juniors | 0 | v | 0 | Petershill | | Replay 2-1 to Petershill |
| 04 Nov 06 | Tranent Juniors | 2 | v | 1 | Sunnybank | | |
| 04 Nov 06 | Maud | 0 | v | 1 | Kirkintilloch Rob Roy | | |
| 04 Nov 06 | Bishopmill United | 0 | v | 1 | Aberdeen East End | | |
| 04 Nov 06 | Kilbirnie Ladeside | 4 | v | 3 | Glasgow Perthshire | | |
| 04 Nov 06 | Fauldhouse United | 0 | v | 0 | Cumbernauld United | | Replay 0-0, Fauldhouse win 4-2 on penalties |
| 04 Nov 06 | Dunipace | 0 | v | 1 | Rutherglen Glencairn | | |
| 04 Nov 06 | Yoker Athletic | 1 | v | 0 | Ardrossan Winton Rovers | | |
| 04 Nov 06 | Lugar Boswell Thistle | 3 | v | 0 | Parkvale | | |
| 04 Nov 06 | Dundee East Craigie | 2 | v | 2 | Buchanhaven Hearts | | Replay 2-1 to East Craigie |
| 04 Nov 06 | Thornton Hibs | 1 | v | 7 | Dundonald Bluebell | | |
| 04 Nov 06 | Stevenston Ardeer Thistle | 0 | v | 1 | Ellon United | | |
| 04 Nov 06 | St. Roch's | 6 | v | 3 | Lossiemouth United | | |
