The action got under way on Friday night as Dalkeith Thistle hosted Broxburn Athletic and Tynecastle entertained Hill of Beath Hawthorn.
The BBC cameras and the Scottish Cup trophy were in Dalkeith, with pyrotechnics and a large support present for the launch of the 2024/2025 competition. Broxburn spoiled their hosts' big night, however, with Errol Douglas bagging a hat-trick Athletic secured a 4-0 win.
It was also a triumph for the away side in the other of Friday's matches, with the Haws' front three of Dan Watt, John Smith and Jordan Tosh all on target in a 3-2 win over Tynecastle at Meggetland.
The other 28 ties were played on Saturday in an action-packed afternoon of Scottish Cup action.
Highland League and Lowland League leaders both made it through unscathed, with Brechin City winning 1-0 against Newtongrange Star and Caledonian Braves winning 2-0 away to Unversity of Stirling.
Fraserburgh were the day's high-scorers, winning 8-0 at home to Rothes, while Sauchie Juniors enjoyed a memorable 6-0 away win against Strathspey Thistle.
Three matches went the distance and Inverurie Loco Works, Huntly and Keith all prevailed on spot-kicks against Dundonald Bluebells, Wick Academy and Camelon Juniors, respectively.
West of Scotland Premier League side Auchinleck Talbot produced one of the results of the day, winning 2-1 away to Lowland League outfit Albion Rovers. Alex Nimmo and Connor Boyd grabbed their goals, with Adam Fernies' late strike proving a consolation.
Another surprise result came at the Falkirk Stadium as Midlands League side Lochee United defeated another Lowland League team, East Stirlingshire, winning 3-2.
Cupar Hearts - back-to-back Scottish Amatuer Cup winners - were competing in the Scottish Cup First Round for the first time and they took a dream lead within a minute against Berwick Rangers. The Fifers were unable to hang on to the lead, however, and Player of the Match Jonny Devers bagged a brace as Rangers eventually triumphed 4-2.
Bo'ness Athletic fared better on their First Round debut, winning 4-1 away to Dunbar United. Ross Nimmo and Sandy Cunningham were both at the double, while goalkeeper Fraser Currid saved a penalty on a memorable Saturday for Athletic, who have already come through two Preliminary Rounds to reach this stage.
The full list of results is listed at the bottom of the page.
Scottish Gas Men's Scottish Cup Second Round Draw
Jack Cochrane and Jordan Mackay from Scottish indie rock band, The Snuts, will join host Gordon Duncan for the Round Two draw, which will take place at 5pm at Hampden Park, live on Scottish Cup social media channels.
Scottish Gas Men's Scottish Cup First Round Results
Albion Rovers 1-2 Auchinleck Talbot
Berwick Rangers 4-2 Cupar Hearts
Brechin City 1-0 Newtongrange Star
Broomhill 0-1 Turriff United
Brora Rangers 2-1 Cumnock Juniors
Buckie Thistle 2-1 Carluke Rovers
Burntisland Shipyard 1-4 Irvine Meadow XI
Clachnacuddin 3-1 Nairn County
Clydebank 3-1 Girvan
Cowdenbeath 3-2 Rutherglen Glencairn
Cumbernauld Colts 0-3 Civil Service Strollers
Dalkeith Thistle 0-4 Broxburn Athletic
Deveronvale 1-4 Bo’ness United
Dunbar United 1-4 Bo’ness Athletic
Dundee North End 4-0 Tranent
Dundonald Bluebell 1-1 Inverurie Loco Works (Inverurie Loco Works win 3-0 on pens)
East Kilbride 2-0 Gretna 2008
East Stirlingshire 2-3 Lochee United
Formartine United 2-0 Whitehill Welfare
Forres Mechanics 1-2 Jeanfield Swifts
Fraserburgh 8-0 Rothes
Gala Fairydean Rovers 0-1 Banks O’Dee
Huntly 2-2 Wick Academy (Huntly win 5-3 on pens)
Keith 1-1 Camelon Juniors (Keith win 4-3 on pens)
Linlithgow Rose 3-1 Lossiemouth
Musselburgh Athletic 1-0 (after extra-time) Darvel
Strathspey Thistle 0-6 Sauchie Juniors
Threave Rovers 6-1 Vale of Leithen
Tynecastle 2-3 Hill of Beath Hawthorn
University of Stirling 0-2 Caledonian Braves